购买Beacon designer软件 本地化服务
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关 键 词:designer软件,购买Beacon
行 业:IT 软件 地理信息系统
发布时间:2022-01-26
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HRMA试验设计
Beacon Designer为突变检测提供了一个综合解决方案。高分辨率熔解分析(HRMA)是一种比基于前列腺的基因型分析更具有成本效益的方法。该程序使用专有算法,可以设计出检测突变的佳引物。
高分辨率的熔解分析引物被设计成侧翼感兴趣突变,产生具有可检测的熔融温度变化的短可能的扩增子。引物设计避免了模板二级结构,保证有效引物延伸。设计的HRMA引物可以在NCBI的核苷酸数据库中进行搜索,以检验其特。还可以对预先设计或发布的引物进行分析。
The other unique feature of this tool is the automatic avoidance of the secondary structures that can prevent the SYBR® Green primer extension. The template forms a loop in the region of the secondary structures and this loop prevents the PCR product formation.
Salient Features Supported for MethyLight Assays in Beacon Designer™
a) Prediction of CpG islands in the DNA sequence.
b) Design methyl specific primers and TaqMan® probes.
c) Design suitable control primers and TaqMan® probes for corresponding unmethylated and untreated sequences.
d) Analyze pre-designed/published primers and/or TaqMan® probes and design of compatible oligos.
e) Primers and TaqMan® probes are designed by avoiding regions of homology identified by automatically interpreting BLAST search results.
MethyLight Assay Design for DNA Methylation Profiling
MethyLight assay, a TaqMan® probes based methylation profiling technique, can be effectively used for DNA methylation profiling, measure DNA methylation or detect methylated alleles in presence of the unmethylated ones. Due to the specificity of TaqMan® based assays, it is much more accurate than the end point detection assay such as methyl specific PCR (MSP).
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