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关 键 词:AlleleID云盘
行 业:IT 软件 实用工具软件
发布时间:2021-12-27
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Real Time PCR Primer and Probe Design with AlleleID® & Beacon Designer™
With ClustalW multiple sequence alignment at its core, AlleleID® can be used to design species identification/cross species probes for microarrays or real time PCR including SYBR® Green, TaqMan® MGB, TaqMan® probes, Molecular Beacons and real time PCR primers.
Modern Techniques for Cross Species Detection
Real time PCR and microarrays offer a rapid, sensitive, specific and quantitative tool for the diagnosis of pathogens. Diseases are not readily distinguished based on symptoms when there is more than one pathogen in the sample. Microscopic identification requires culturing on specialized media for days to weeks. Recently, real time PCR assays were successfully used in the detection of fungal pathogens such as Rhizoctonia, Pythium, and Fusarium.
Designing a cross species assay involves the use of a multiple sequence alignment tool to identify conserved stretches of DNA and the design of a hybridization probe for detecting it. Recently there have been advances in micro-organism identification at the taxonomical level using molecular level identification techniques such as real time PCR and microarrays.
Minimal Set with Unique Primers: Choosing the Minimal set option, AlleleID® designs the smallest number of probes required to identify the sequences. The Minimal set will generally contain fewer probes than the number of sequences to be identified, and a unique combination of probes identifies each sequence. The aim of such an assay is to minimize the synthesis and overall assay costs.
Species Identification: Minimal Set with Unique Primers
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