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关 键 词：多肽合成；多肽偶联；多肽荧光修饰合成
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发布时间：2015-03-14
N末端的生物素标记 Wash 0.1 mmol resin with DMF. Dissolve 0.244 g (+)-biotin (1 mmol, MW 244.3) in 5 mL DMF-DMSO (1:1) solution. A little warming is necessary. Add 2.1 mL 0.45 M HBTU/HOBt solution and 0.3 mL DIEA to the solution prepared in step 2. Add the activated biotin solution to the resin and let stir overnight. Check resin to make sure coupling is complete as evidenced by negative ninhydrin test (colorless). Wash resin with DMF-DMSO (1:1) (2x) to remove excess (+)-biotin. Wash resin with DMF (2x) and DCM (2x). Let the resin dry before proceeding to cleavage. 在2-氯TRT树脂上挂第一个FMOC氨基酸的方法 Weigh 10 g 2-chlorotrityl chloride resin (15 mmol) in a reaction vessel, wash with DMF (2x), swell the resin in 50 mL DMF for 10 min, drain vessel. Weigh 10 mmol Fmoc-amino acid in a test tube, dissolve Fmoc-amino acid in 40 mL DMF, transfer the solution into the reaction vessel above, add 8.7 mL DIEA (50 mmol), swirl mixture for 30 min at room temperature. Add 5 mL methanol into the reaction vessel and swirl for 5 min. Drain and wash with DMF (5x). Check substitution. Aproduct. SomeShake the resin in 3% TFA for 10 min. Repeat step 3. Wash resin with DCM (3x), DMF (3x), isopropanol (3x), and DCM (3x). Let the resin dry in air. 多肽标记FITC方法 Reagents: FITC Fmoc-e-Ahx-OH Couple Fmoc-e-Ahx-OH to the amino terminal of the peptide-resin using standard coupling conditions. "De-Fmoc" with piperidine using the standard 20% piperidine procedure. Wash resin with DMF (3-4x). Swell resin with DCM and drain. Prepare solution of 1.1 equivalent of FITC in pyridine/DMF/DCM (12:7:5). Use just enough solution to form a slurry with the resin. Do not use too much solution since the rate of the reaction is proportionate to the concentration of the solution. Add the solution prepared in step 2 to the resin. Let mix overnight. Check the completion of the reaction using ninhydrin test. If the coupling of FITC to the amino group is not complete, ninhydrin test will give a blue color. Repeat the coupling with FITC (steps 5-7) if necessary. Wash resin with DMF (2x), isopropanol (2x), and DCM (2x). 欢迎来订购多肽 手机号码： Q Q号： 公司电话： 027-87687993 公司传真： 027-87687992 电子邮件： 公司网址
