供应大鼠苗条素受体(LR/Ob-R)ELISA试剂盒

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产品规格：96T/48T

产品数量：100 个

包装说明：盒（按客户需求）

关 键 词：ELISA试剂盒,ELISA试剂盒说明书,大鼠elisa试剂盒

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发布时间：2015-11-24

大鼠苗条素受体(LR/Ob-R)ELISA试剂盒
大鼠苗条素受体(LR/Ob-R)ELISA试剂盒说明书
上海远慕生物做实验试剂，专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂！欢迎来电咨询订购：Elisa试剂盒现货低价！特价促销！
Rat Leptin receptor,LR/Ob-R ELISA KIT 
产品货号：YM-KJ1631
规格：96T/48T
欢迎来电咨询订购：Elisa试剂盒，大鼠苗条素受体(LR/Ob-R)ELISA检测试剂盒

Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8

Objective: this kit is used to determine the content of serum, plasmaand related liquid samples.

Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without thesample and the enzyme label, and the remaining steps are the same), thesample hole.
3 temperature Education: 37 minutes after the closure of the sealingplate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid withdistilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discardliquid, drying, washing liquid to fill each hole, standing for 30seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gentlyshake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction(at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero airconditioning, in order to measure the hole (OD). Determination should becarried out within 15 minutes after the termination of the liquid.

大鼠苗条素受体(LR/Ob-R)ELISA试剂盒试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%

检测范围：
0.2IU/L - 6IU/L 
温馨提示：不可用于临床治疗。