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发布时间：2010-10-19
When we introduced the Biotin-Avidin System a substantial amplification over the earlier methods was achieved. Avidin is an egg-white derived glycoprotein with an extraordinarily high affinity (affinity constant > 1015 M-1) for biotin. Streptavidin is similar in properties to avidin but has a lower affinity for biotin. Many biotin molecules can be coupled to a protein, enabling the biotinylated protein to bind more than one molecule of avidin. If biotinylation is performed under gentle conditions, the biological activity of the protein can be preserved. By covalently linking avidin with different ligands such as fluorochromes, enzymes or EM markers, what we have termed the Biotin-Avidin System can be utilized to study a wide variety of biological structures and processes. The Biotin-Avidin System has proven to be particularly useful in the detection and localization of antigens, glycoconjugates, and nucleic acids by employing biotinylated antibodies, lectins, or nucleic acid probes. Biotin Strept avidin Systems 1.抗亲和素试剂 Biotinylated Anti-Avidin D BA-0300 0.5 mg Fluorescein Anti-Avidin D SP-2040 0.5 mg Unconjugated Anti-Avidin D SP-2000 1 mg 2.抗生物素试剂 Anti-Biotin, Made in Goat, Agarose Bound SP-3030 2 ml Anti-Biotin, Made in Goat, Alkaline Phosphatase Conjugated SP-3020 1 ml Anti-Biotin, Made in Goat, Fluorescein Conjugated SP-3040 0.5 mg Anti-Biotin, Made in Goat, Peroxidase Conjugated SP-3010 1 mg Anti-Biotin, Made in Goat, Unconjugated SP-3000 1 mg Anti-Biotin-M, Mouse Monoclonal Antibody MB-9100 1 ml 3.抗链酶亲和素 Biotinylated Anti-Streptavidin BA-0500 0.5 mg Fluorescein Anti-Streptavidin SP-4040 0.5 mg Unconjugated Anti-Streptavidin SP-4000 1 mg Avidin D A-2000 10 mg Avidin DN A-3100 1 mg Alkaline Phosphatase Avidin D A-2100 100 U Beta-Galactosidase Avidin D A-2300 100 U Horseradish Peroxidase Avidin D, Concentrate A-2004 5 mg R.T.U. Horseradish Peroxidase Avidin D A-2704 100 ml 4.生物素化的试剂 Biotinylated Agarose B-2011 5 ml Biotinylated Alkaline Phosphatase B-2005 1 mg Biotinylated Anti-Avidin D BA-0300 0.5 mg Biotinylated Anti-DNP BA-0603 0.5 mg Biotinylated Anti-Fluorescein BA-0601 0.5 mg Biotinylated Anti-Human IgA, alpha chain specific, made in goat BA-3030 0.5 mg Biotinylated Anti-Human IgE, epsilon chain specific, made in goat BA-3040 0.5 mg
