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| 表达宿主 | HEK293 cells |
|---|---|
| 描述 | Active Recombinant Human IL-10 R alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His22-Asn235) of human IL-10 R alpha (Accession #NP_001549.2) fused with and a hFc tag at the C-terminus. |
| 标签 | C-hFC |
| 内毒素 | <0.1EU/ug |
| 生物活性 | Measured by its binding ability in a functional ELISA.Immobilized Human IL10 (Catalog: RP00093) at 2 μg/mL (100 μL/well) can bind Human IL10RA (Catalog: RP01647) with a linear range of 0.1-8.7 ng/mL. |
| 制剂 | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| 储存 | Store the lyophilized protein at -20°C to -80°C for 12 months. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| 复溶 | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
